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CYT-304

重組人可溶性CD4 （rsCD4）

2μg/10μg/1mg

Prospec

Background:
	CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MHC （Major Histocompatibility Complex） Class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as HIV receptors, binding directly to the envelope protein gp120 on HIV. Thymocytes which are CD4 （+）and CD8 （ - ）produce factors such as IL-4 , IL-5 ,IL-10 and IFN-gamma
	Description :
	Recombinant Human sCD4 produced in Baculovirus is a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 40,000 Dalton. As a result of glycosilation the sCD4 migrates as a 46,000 dalton protein on standard SDS-PAGE. Detected By Western Imunno Blots anti cd4 monoclonal antibody. Recombinant soluble CD4 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered colorless liquid formulation.
	Formulation:
	Recombinant Human Soluble CD-4 solution contains 10mM sodium phosphate buffer pH-7.2, 300mM sodium chloride and 0.01% tween.
	Stability:
	rHusCD4 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 90.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s Recombinant Human Soluble CD-4 is fully biologically active when compared to standard. Immune reactive in ELISA using monoclonal anti CD4 in gpl2O capture ELISA assays.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of soluble CD4.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of CD4 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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