Recombinant Protocadherin Beta 15 (PCDHb15)  

原鈣黏素β15(PCDHb15)重組蛋白

[ PROPERTIES ]

Residues: Val35~Leu347

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9Y5E8

Host:E. coli

Subcellular Location: Cell membrane; Single-passtype I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.5

Predicted Molecular Mass: 38.2kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

VMEETE RGSFVANLAN DLGLGVGELA ERGARVVSED NEQGLQLDLQTGQLILNEKLDREKLCGPTE PCIMHFQVLL KKPLEVFRAE LLVTDINDHS PEFPEREMTL KIPETSSLGTVFPLKKARDL DVGSNNVQNY NISPNSHFHV STRTRGDGRK YPELVLDTEL DREEQAELRLTLTAVDGGSP PRSGTVQILI LVLDANDNAP EFVQALYEVQ VPENSPVGSL VVKVSARDLDTGTNGEISYS LYYSSQEIDK PFELSSLSGE IRLIKKLDFE TMSSYDLDIE ASDGGGLSGKCSVSVKVLDV NDNFPEL

[  IDENTIFICATION  ]

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