NR8383大鼠肺泡巨噬細(xì)胞（
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大鼠肺泡巨噬細(xì)胞NR8383
| 屬 | 大鼠 |
| 別稱 | NR-8383; NR 8383; NR8383.1; AgC11x3A; Normal Rat, August 3, 1983 |
| 組織來(lái)源 | 肺；肺泡 |
| 疾病 | 自發(fā)永生細(xì)胞系 |
| 傳代比例/細(xì)胞消化 | 1:2傳代,懸浮部分離心收集(1000RPM,5分鐘) ，貼壁部分消化1-2分鐘 |
| 培養(yǎng)基配置 | Ham's F-12K培養(yǎng)基; 20%胎牛血清；1%雙抗 |
| 簡(jiǎn)介 | NR8383細(xì)胞來(lái)源于肺灌洗時(shí)的正常大鼠肺泡巨噬細(xì)胞 ，NR8383細(xì)胞在Gerbil肺細(xì)胞連續(xù)培養(yǎng)液存在下培養(yǎng)了8-9個(gè) 月。隨后 ，NR8383細(xì)胞不再需要外源生長(zhǎng)因子。通過(guò)有限稀釋法 ，從單個(gè)細(xì)胞克隆并亞克隆NR8383細(xì)胞 ，并3次用 軟瓊脂亞克隆。NR8383細(xì)胞表現(xiàn)出巨噬細(xì)胞的特性：吞噬酵母多糖和銅綠、非特異性脂酶活性、Fc受體、氧化降 解；分泌IL-1、TNF-β和IL-6 ，可重復(fù)地響應(yīng)外源生長(zhǎng)因子。NR8383細(xì)胞響應(yīng) ，分泌TNF-β前體。 NR8383細(xì)胞在刺激下 ，TNF-β mRNA表達(dá)也上升。NR8383細(xì)胞對(duì)內(nèi)毒素敏感 ，1-10ng/ml的LPS水平抑 制增生達(dá)50%。即使達(dá)到0.001mg/ml的水平 ，LPS抑制還是無(wú)毒且在后續(xù)過(guò)程中可逆的。NR8383細(xì)胞提供了高響 應(yīng)的肺泡巨噬細(xì)胞的均一來(lái)源 ，可以用于體外研究巨響細(xì)胞相關(guān)活性 |
| 形態(tài) | 巨噬細(xì)胞樣 |
| 生長(zhǎng)特征 | 貼壁 ，懸浮混合生長(zhǎng) |
| 倍增時(shí)間 | 每周 2 至 3 次 |
| 培養(yǎng)條件 | 氣相：空氣 ，95% ；二氧化碳 ，5%。 溫度：37攝氏度 ，培養(yǎng)箱濕度為70%-80%。 |
| 凍存條件 | 凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號(hào)JY-H040 |
| 保藏機(jī)構(gòu) | ATCC; CRL-2192 |
| 備注 | 該細(xì)胞為半懸浮和半貼壁細(xì)胞 ，懸浮細(xì)胞離心收集 ，貼壁細(xì)胞消化處理 |
| 產(chǎn)品使用 | 于科學(xué)研究 ，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。 |
cowpea-wheat crop sequence. Appl. Soil Ecol. 157, 103764
Galvez, A., Sinicco, T., Cayuela, g.L., gingorance, g.D., Fornasier, F., gondini, C.,
atientswithAISofditerentseverities were compared , andthecorrelationbetween serum OccludinlevelsandNIHSSscoresin p atientswith AIS was
as a p redictorofAISoccurrence , hemorrha g ictransformation and p oor p ro g nosis .
Keywords:Acuteischemicstroke ; Occludin; Bloodbrainbarrier
Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60?min 6x/week for 12?weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD bａnd plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.
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