UM-UC-3人膀胱移行細胞癌
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人膀胱移行細胞癌UM-UC-3
| 種屬 | 人 |
| 別稱 | UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3 |
| 組織來源 | 膀胱 |
| 疾病 | 移行細胞癌 |
| 傳代比例/細胞消化 | 1:2傳代 ，消化2-3分鐘 |
| 培養(yǎng)基配置 | MEM培養(yǎng)基；10%胎牛血清；1%雙抗 |
| 簡介 | Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) ａnd others. The X and N3 had single copy per cell, and others were generally two or three copies per cell. |
| 形態(tài) | 上皮細胞樣 |
| 生長特征 | 貼壁生長 |
| 倍增時間 | 每周 2 至 3 次 |
| 致瘤性 | Yes, Tumors developed within 21 days at 99% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| STR | Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10 vWA: 17 |
| 培養(yǎng)條件 | 氣相 ：空氣 ，95% ；二氧化碳 ，5%。 溫度 ：37攝氏度 ，培養(yǎng)箱濕度為70%-80%。 |
| 凍存條件 | 凍存液 ：90%FBS ，DMSO 10%, 或使用非程序凍存液 ：貨號JY-H040 |
| 保藏機構 | ATCC; CRL-1749 |
| 產品使用 | 于科學研究 ，不可作為動物或人類疾病的治療產品使用。 |
Compared with the control group，the expression levels of Afamin and DBP in serum of the POI group were significantly increased (P＜0．05) ; the expression levels of serum Afamin and DBP were positively correlated with FSH and E2 (P＜ 0．05) ，but negatively correlated with AMH (P＜0. 05) ;
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