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CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞

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型號 CCL-9.1
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更新時(shí)間 2025/8/14 12:17:58
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CCL-9.1 NCTC 1469 ATCC 細(xì)胞

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上海復(fù)祥生物科技有限公司是一家專業(yè)從事原代細(xì)胞、細(xì)胞系；菌種；及細(xì)胞因子相關(guān)產(chǎn)品的銷售企業(yè)。公司建有自己的細(xì)胞庫和菌種庫。細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件，公司引進(jìn)ATCC細(xì)胞500余種，ATCC菌種800余種。并和國內(nèi)各大保藏中心有密切聯(lián)系，細(xì)胞種類齊全，質(zhì)量保證！是生命科學(xué)領(lǐng)域*的一部分！

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ATCC 細(xì)胞,細(xì)胞系,細(xì)胞株,腫瘤細(xì)胞,細(xì)胞,ATCC 菌種，CMCC 菌種，標(biāo)準(zhǔn)菌株，質(zhì)控菌種，微生物菌種，菌株，菌種

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供貨周期	一周	規(guī)格	T25
貨號	NCTC 1469 小	應(yīng)用領(lǐng)域	醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
主要用途	科學(xué)研究

CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞 的詳細(xì)介紹

CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞

ATCC^® Number:

CCL-9.1™

Price:

$329.00

Designations:	NCTC clone 1469 [derivative of NCTC 721]
Depositors:	VJ Evans
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus （mouse）
Morphology:	epithelial
Source:	Organ: liver Strain: C3H/An Disease: normal
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-9.1
Isolation:	Isolation date: April, 1952
Virus Susceptibility:	Vesicular stomatitis virus Human poliovirus 1
Antigen Expression:	H-2k
Cytogenetic Analysis:	modal number = 41; range = 38 to 86. Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with salites.
Age:	newborn
Gender:	male
Comments:	Tested and found negative for ectromelia virus （mousepox）.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C
Subculturing:	Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks. A standard trypsinizaton may be used if desired. The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination. It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures. *Subction Ratio:** A subction ratio of 1:2 to 1:4 is recommended Medium Renewal:* 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2040
References:	22167: Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690 26070: Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026 26074: . . J. Natl. Cancer Inst. 23: 823, 1959. 26076: . . J. Natl. Cancer Inst. 27: 29, 1961. 26078: Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589 26079: . . Fed. Proc. 17: 967, 1958. 26081: Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338

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