国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

官方微信|手機版

產(chǎn)品展廳

產(chǎn)品求購企業(yè)資訊會展

發(fā)布詢價單

化工儀器網(wǎng)>產(chǎn)品展廳>試劑標物>行業(yè)專用試劑>生物試劑>CL-173 3T3-L1 小鼠脂肪細胞

CL-173 3T3-L1 小鼠脂肪細胞

參考價 1
訂貨量 ≥1
具體成交價以合同協(xié)議為準
產(chǎn)品標簽

3T3-L1ATCC 細胞

聯(lián)系方式:李經(jīng)理查看聯(lián)系方式

聯(lián)系我們時請說明是化工儀器網(wǎng)上看到的信息,謝謝!


上海復(fù)祥生物科技有限公司是一家專業(yè)從事原代細胞、細胞系;菌種;及細胞因子相關(guān)產(chǎn)品的銷售企業(yè)。公司建有自己的細胞庫和菌種庫。細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件,公司引進ATCC細胞500余種,ATCC菌種800余種。并和國內(nèi)各大保藏中心有密切聯(lián)系,細胞種類齊全,質(zhì)量保證!是生命科學(xué)領(lǐng)域*的一部分!

公司秉承:客戶至上的原則,為所有客戶提供*質(zhì)的產(chǎn)品和服務(wù)!

為我國的生命科學(xué)事業(yè)增添一份力量!



ATCC 細胞,細胞系,細胞株,腫瘤細胞,細胞,ATCC 菌種,CMCC 菌種,標準菌株,質(zhì)控菌種,微生物菌種,菌株,菌種

供貨周期 一周 規(guī)格 T25
貨號 3T3-L1 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
主要用途 科學(xué)研究

3T3-L1 小鼠脂肪細胞 的詳細介紹

3T3-L1 小鼠脂肪細胞

ATCC® Number:CL-173™  Price:
Designations:3T3-L1

Depositors:Massachusetts Institute of Technology

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast
3T3-L1 小鼠脂肪細胞


Source:Organ: embryo
Cell Type: fibroblast


Cellular Products:triglycerides [3491]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may CCbe required for the transfer of this ATCC material. Anyone purchasing AT material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)



Receptors:insulin, expressed

Reversenegative

Age:embryo

Comments:L1 is a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation. The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090].

Tested and found negative for ectromelia virus (mousepox).

This line is also designated as ATCC CCL-92.1.ATCC CL-173 was deposited in 1974 without passage number information from the depositor. At the time of submission, ATCC prepared approximay 30 vials of seed stock at about 4 passages beyond the original depositor material (passage number: unknown +4).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final ation of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The serum used is important in culturing this line. Calf serum is recommended and not fetal bovine serum.


Subculturing:Protocol: Never allow culture to become compley confluent.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

    The recommended inoculum is 2 to 3 X 10(3) cells/sq. cm. Subculture before cultures become 70 to 80% confluent or when cells reach 5 to 6 X10(4) viable cells/sq. cm.

  6. Incubate cultures at 37C.


Interval: Every three days
Medium Renewal: 2 to 3 times per week


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:14 hrs

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

formerly distributed as:ATCC CCL-92.1

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

Recommended serum: ATCC 30-2030



References:886: Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 4426090

3491: Green H. Triglyceride-accumulating clonal cell line. US Patent 4,003,789 dated Jan 18 1977

32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260






















化工儀器網(wǎng)

采購商登錄
記住賬號    找回密碼
沒有賬號?免費注冊

提示

×

*您想獲取產(chǎn)品的資料:

以上可多選,勾選其他,可自行輸入要求

個人信息:

溫馨提示

該企業(yè)已關(guān)閉在線交流功能

阿鲁科尔沁旗| 上林县| 湖北省| 阿荣旗| 合水县| 高尔夫| 会理县| 库车县| 张掖市| 麻城市| 新野县| 佛学| 台湾省| 光山县| 武强县| 富民县| 新余市| 贵州省| 四平市| 许昌县| 四川省| 香格里拉县| 射阳县| 绥阳县| 六盘水市| 丰宁| 永安市| 台前县| 天门市| 舞钢市| 嵊泗县| 滕州市| 康定县| 惠来县| 莒南县| 宿松县| 平山县| 长治市| 尚义县| 罗甸县| 桦川县|