CRL-1715 TM4 正常小鼠睪丸Leydig細胞

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CRL-1715 TM4 ATCC 細胞

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上海復祥生物科技有限公司是一家專業(yè)從事原代細胞、細胞系；菌種；及細胞因子相關(guān)產(chǎn)品的銷售企業(yè)。公司建有自己的細胞庫和菌種庫。細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件，公司引進ATCC細胞500余種，ATCC菌種800余種。并和國內(nèi)各大保藏中心有密切聯(lián)系，細胞種類齊全，質(zhì)量保證！是生命科學領(lǐng)域*的一部分！

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ATCC 細胞,細胞系,細胞株,腫瘤細胞,細胞,ATCC 菌種，CMCC 菌種，標準菌株，質(zhì)控菌種，微生物菌種，菌株，菌種

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供貨周期	一周	規(guī)格	T25
貨號	CRL-1715	應用領(lǐng)域	醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
主要用途	科學研究

CRL-1715 TM4 正常小鼠睪丸Leydig細胞 的詳細介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細胞

ATCC^® Number:

CRL-1715™

Price:

$329.00

Designations:	TM4
Depositors:	JP Mather
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus （mouse）
Morphology:	epithelial
Source:	Organ: testis Disease: normal Cell Type: Sertoli cell;
Cellular Products:	retinol binding protein tissue plasminogen activator transferrin
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host （Roche FuGENE® Transfection Reagents）
Receptors:	follicle stimulating hormone （FSH）, expressed androgen receptor, expressed progesterone receptor, expressed
Tumorigenic:	No
Antigen Expression:	H-Y antigen; Mus musculus, expressed
Age:	11 to 13 days
Gender:	male
Comments:	The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone （LH）. The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid. Tested and found negative for ectromelia virus （mousepox）.
Propagation:	ATCC complete growth medium: A 1:1 mixture of Ham'S F12 medium and Dulbecco's modified Eagle's medium with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5% Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell supension to new culture vessels. Incubate cultures at 37C. *Subction Ratio:** A subction ratio of 1:10 to 1:50 is recommended Medium Renewal:* Every 3 to 4 days
Preservation:	Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2006 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040
References:	1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781 1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561 1184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259: 3117-3123, 1984. PubMed: 6538197 26150: Mather JP, Phillips DM. Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture. J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874

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