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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標(biāo)物>行業(yè)專用試劑>生物試劑> MC 3T3-E1 小鼠成骨細(xì)胞

MC 3T3-E1 小鼠成骨細(xì)胞

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MC 3T3-E1

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上海復(fù)祥生物科技有限公司是一家專業(yè)從事原代細(xì)胞、細(xì)胞系;菌種;及細(xì)胞因子相關(guān)產(chǎn)品的銷售企業(yè)。公司建有自己的細(xì)胞庫(kù)和菌種庫(kù)。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件,公司引進(jìn)ATCC細(xì)胞500余種,ATCC菌種800余種。并和國(guó)內(nèi)各大保藏中心有密切聯(lián)系,細(xì)胞種類齊全,質(zhì)量保證!是生命科學(xué)領(lǐng)域*的一部分!

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ATCC 細(xì)胞,細(xì)胞系,細(xì)胞株,腫瘤細(xì)胞,細(xì)胞,ATCC 菌種,CMCC 菌種,標(biāo)準(zhǔn)菌株,質(zhì)控菌種,微生物菌種,菌株,菌種

供貨周期 一周 規(guī)格 T25
貨號(hào) MC 3T3-E1 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
主要用途 科學(xué)研究

MC 3T3-E1 小鼠成骨細(xì)胞 的詳細(xì)介紹

MC 3T3-E1 小鼠成骨細(xì)胞

ATCC® Number:CRL-2593™  Price:$324.00
Designatio
Level:1
Shipped:frozen
ns:
MC3T3-E1 Subclone 4

Depositors:RT Franceschi

Biosafety


Medium & Serum:See Propagation

Growth Properties:adherent

Organism:M
se)
Morphology:fibroblast
us musculus (mou


Source:Organ: bone
Strain: C57BL/6
Tissue: calvaria
Cell Type: preosteoblast;


Cellular Products:collagen [51540]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Tumorigenic:Yes

Age:newborn newborn

Comments:A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 (ATCC CRL-2593) and the MC3T3 Subclone 14 (ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix (ECM) after 10 days [PubMed ID: 10352097].

The MC3T3 Subclone 24 (ATCC CRL-2595) and the MC3T3 Subclone 30 (ATCC CRL-2596) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14 [PubMed ID: 10352097].

Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor.S ubclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor [PubMed ID: 10352097].























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