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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標物>行業(yè)專用試劑>生物試劑>CRL-1378 RBL-1 大鼠嗜堿性粒細胞白血病細胞

CRL-1378 RBL-1 大鼠嗜堿性粒細胞白血病細胞

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  • 公司名稱 上海復祥生物科技有限公司
  • 品牌 ATCC
  • 型號 CRL-1378
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  • 更新時間 2025/8/14 12:10:33
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ATCC 細胞

聯(lián)系方式:李經(jīng)理查看聯(lián)系方式

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上海復祥生物科技有限公司是一家專業(yè)從事原代細胞、細胞系;菌種;及細胞因子相關(guān)產(chǎn)品的銷售企業(yè)。公司建有自己的細胞庫和菌種庫。細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件,公司引進ATCC細胞500余種,ATCC菌種800余種。并和國內(nèi)各大保藏中心有密切聯(lián)系,細胞種類齊全,質(zhì)量保證!是生命科學領(lǐng)域*的一部分!

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ATCC 細胞,細胞系,細胞株,腫瘤細胞,細胞,ATCC 菌種,CMCC 菌種,標準菌株,質(zhì)控菌種,微生物菌種,菌株,菌種

供貨周期 一周 規(guī)格 T25
應用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè) 主要用途 科學研究

CRL-1378 RBL-1 大鼠嗜堿性粒細胞白血病細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件

CRL-1378 RBL-1 大鼠嗜堿性粒細胞白血病細胞 的詳細介紹

CRL-1378

ATCC® Number:CRL-1378™  Price:$329.00
Designations:RBL-1

Depositors:H Metzger, C Isersky

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Rattus norvegicus (rat)

Morphology:lymphoblast


Source:Organ: peripheral blood
Strain: Wistar
Disease: leukemia
Cell Type: basophil; chemically induced


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Nucleofection technology from Lonza)

Receptors:FcERI (Fc of IgE)

Comments:The line exhibits various characteristics of basophil differentiation including surface receptors for IgE. It was shown to not release histamine by an IgE mediated system. PubMed: 6166481

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: Cultures can be maintained by the addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 cells/ml and 1 X 10 exp6 /ml..
Medium Renewal: Add medium as cell density increases


Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:1232: Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630

22475: Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481

22638: Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.

























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