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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標(biāo)物>行業(yè)專用試劑>生物試劑>CRL-1708 QT6 鵪鶉纖維肉瘤細胞

CRL-1708 QT6 鵪鶉纖維肉瘤細胞

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ATCC 細胞鵪鶉纖維肉瘤細胞

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ATCC 細胞,細胞系,細胞株,腫瘤細胞,細胞,ATCC 菌種，CMCC 菌種，標(biāo)準(zhǔn)菌株，質(zhì)控菌種，微生物菌種，菌株，菌種

供貨周期 一周 規(guī)格 T25
貨號 QT6 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥
主要用途 科學(xué)研究

CRL-1708 QT6 鵪鶉纖維肉瘤細胞的詳細介紹

CRL-1708 QT6 鵪鶉纖維肉瘤細胞

ATCC® Number:CRL-1708™  Price:$438.00
Designations:QT6

Depositors:C Moscovici

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Coturnix coturnix japonica （quail, Japanese）

Morphology:fibroblast


Source:Disease: fibrosarcoma
Cell Type: methylcholanthrene induced

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host （Roche FuGENE® Transfection Reagents）

Virus Susceptibility:Rous sarcoma virus

Avian encephalomyelitis virus

Rabies virus

Marek's disease virus



Tumorigenic:Yes

Propagation:ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Temperature: 37.0°C

Subculturing:Protocol: Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximay 75% confluency. Use of 0.5% polyvinylpyrrolidone （PVP） in atrypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.Note: When subcultures are prepared using pipetting or mechanical means thecells adhere lightly to the flask substrate and exhibit a tendency toward clumping.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week

Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase

Related Products:Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004

recommended serum:ATCC 30-2030



References:22385: Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

CRL-1708




















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