ProPacIMAC蛋白質(zhì)和羧氨酸色譜柱 ProPacIMAC蛋白質(zhì)和羧氨酸色譜柱
- 公司名稱 上海普譽(yù)科貿(mào)有限公司
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- 型號(hào) ProPacIMAC蛋白質(zhì)和羧氨酸色譜柱
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- 更新時(shí)間 2018/4/20 4:38:07
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ProPac IMAC 蛋白質(zhì)和羧氨酸色譜柱 概述: The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2–12 and is compatible with commonly used protein purification reagents （non-ionic detergents, reducing agents）. It can be used under native or denaturing HPLC conditions The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column （an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column）.
Analytical Column for Immobilized Metal Affinity Chromatography
The ProPac® IMAC-10 is an analytical HPLC column packed with 10 µm, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly（IDA） chains. The poly（IDA） grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power.
規(guī)格:
ProPac IMAC Columns Specifications Maximum Operating Pressure <3000 psi （21 MPa） Temperature Range <60oC Recommended Temperature: Controlled ambient Typical Operating Conditions <600 psi at 1.0 mL/min （4 × 250mm） Recommended flow rate: 1.0 mL/min Phase Compatibility pH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl Resin Compostion 10 µm diameter nonporous polystyrene divinylbenzene substrate （55% crosslinking） with poly（IDA） grafts. Metal Ion Capacity （µmol Cu） >80 mmol Cu （4 × 250 mm analytical column） Chelating Metals Ni, Fe, Cu, others Analytical Column Dimensions 1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm 2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm Loading Column Dimensions 4 × 50 mm, 9 × 50 mm, 9 × 250 mm
訂購(gòu)信息:
Analytical Columns ProPac IMAC-10 Column （1 x 50 mm） 063617 ProPac IMAC-10 Column （2 x 50 mm） 063272 ProPac IMAC-10 Column （4 x 50 mm） 063276 ProPac IMAC-10 Column （9 x 50 mm） 063615 ProPac IMAC-10 Column （2 x 250 mm） 063274 ProPac IMAC-10 Column （4 x 250 mm） 063278 ProPac IMAC-10 Column （9 x 250 mm） 063280 ProPac IMAC-10 Column （22 x 250 mm） 063282 Accessories IMAC Loading Column （4 x 50 mm） 063667 IMAC Loading Column （9 x 50 mm） 063710 IMAC Loading Column （9 x 250 mm） 063718
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