黃曲霉素總量ELISA試劑盒(檢飼料/糧食類，靈敏度0.04ppb,一步法)
一、原理

本試劑盒采用間接競爭ELISA方法，在微孔條上預(yù)包被偶聯(lián)抗原，樣本中的殘留物黃曲素將和微孔條上預(yù)包被的偶聯(lián)抗原競爭抗黃曲毒素抗體，加入酶標(biāo)記物后，用TMB底物顯色，樣本吸光值與其所含殘留物黃曲霉素的含量成負相關(guān)，與標(biāo)準曲線比較即可得出相應(yīng)殘留物黃曲霉素的含量。

二、試劑盒特性
u  試劑盒靈敏度：   0.04ppb
u  孵育溫度：       25℃
u  孵育時間：       30min～15min
u  樣本檢測下限
飼  料·················································2 ppb
花  生················································ 2 ppb
大  米················································ 1.6 ppb
玉  米················································  2 ppb
u  交叉反應(yīng)率
黃曲霉素B1 ················································  100%
黃曲霉素B2 ················································  223%
黃曲霉素G1················································  78.4%
黃曲霉素G2 ················································  125%
u  樣本回收率
飼料、大米、玉米、花生 ··················· 100±30%

三、試劑盒組成
1 微量測試孔 每條8孔，一板12條
2 標(biāo)準液×6瓶
（1ml/瓶） 0ppb 0.04ppb
  0.12ppb 0.36ppb
  1.08ppb 3.24ppb
3 酶標(biāo)記物 7ml 紅色帽
4 抗體工作液 7ml 藍色帽
5 底物A液 7ml 白色帽
6 底物B液 7ml 黑色帽
7 終止液 7ml 黃色帽
8 20X濃縮洗滌液 40ml 白色帽
9 2X濃縮復(fù)溶液 50ml 透明帽

四、樣本前處理及實驗分析步驟
   詳見產(chǎn)品說明書。

五、儲藏條件和保質(zhì)期
1、 儲藏條件：試劑盒于2～8℃保存，不要冷凍。
2、 保 質(zhì) 期：該產(chǎn)品有效期為1年，生產(chǎn)日期見包裝盒。