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北京西美杰科技有限公司
主營(yíng)產(chǎn)品： 生物學(xué)檢測(cè)試劑盒,化學(xué)試劑,蛋白質(zhì)氧化損傷,抗原抗體 |
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聯(lián)系電話
18210960361
公司信息
- 聯(lián)系人：
- 西美杰
- 電話：
- 400-050-4006
- 手機(jī)：
- 18210960361
- 售后電話：
- 010-88597838
- 地址：
- 北京市海淀區(qū)金源時(shí)代商務(wù)中心B座10F
- 郵編：
- 100097
- 網(wǎng)址：
- www.xmjsci.com
[供應(yīng)]A5260,0050-DNA Blood Isolation Spin-Kit（血液DNA提取試劑盒）
- 貨物所在地：
- 北京北京市
- 產(chǎn)地：
- 德國(guó)
- 更新時(shí)間：
- 2025-03-19 21:00:07
- 有效期：
- 2025年3月19日--2025年9月19日
- 已獲點(diǎn)擊：
- 300
【簡(jiǎn)單介紹】DNA Blood Isolation Spin-Kit，A5260,0050，50 Isol
【詳細(xì)說(shuō)明】
| HS-No. | 38220000 | |||
|---|---|---|---|---|
| Storage | RT | |||
| except for Proteinase K - Solution: +4°C | ||||
| • Kit for the DNA isolation from blood cells with spin minicolumns | ||||
| • Application of the DNA in PCR, cloning and sequencing | ||||
| • Maximum sample volume 100 μl | ||||
| • Binding capacity of the column 10 μg DNA | ||||
| • sufficient for 50 and 250 assays, respectively | ||||
| • Isolation of fragments in the range of 20 kb | ||||
The DNA Blood Isolation Spin-Kit is based on the ability of DNA to adsorb to silica surfaces in the presence of high concentrations of chaotropic salts. Blood cells are Iysed with the supplied lysis buffer, which contains chaotropic salts and a non-ionic detergent （TL buffer）. Cell lysis is additionally supported by the high-activity protease Proteinase K. In the next step, the whole sample is loaded onto a minicolumn with a special silica resin. The DNA binds to the silica resin, while contaminants （fragmented proteins, salts, etc.） pass freely through the column. Residual contaminations are removed by a washing step. Pure DNA is eluted with a low salt buffer or water and can be used in the following experiments without additional purification procedures （e.g. precipitation）.
