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pPIC9K
型號 | 載體名稱 | 出品公司 | 載體用途 |
VJI0299 | pPIC9K | Invitrogen | 酵母表達(dá)載體 |
測序引物序列:5'd[GCAAATGGCATTCTGACATCC]3'
測序引物:3'AOX1
The pPIC9K vector carries the kanamycin resistance gene that confers resistance to Geneticin® Reagent in Pichia. Spontaneous generation of multiple insertion events can be identified by resistance to increased levels of Geneticin® Reagent.Pichia transformants are selected on histidine-deficient medium and screened for their level of resistance to Geneticin® Reagent. The ability to grow in high concentrations of Geneticin® indicates that multiple copies of the kanamycin resistance gene and the gene of interest are integrated into the genome. The pPIC9K vector directs secretion of expressed proteins.
