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上海起發(fā)實驗試劑有限公司
中級會員·16年
- 聯(lián)系人：
- 楊經(jīng)理
- 電話：
- 021-50724187 021-50724961
- 手機：
- 15921799099
- 傳真：
- 021-50724961
- 地址：
- 上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實驗試劑有限公司
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- www.qfbio.com
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Santa Cruz，Santa Cruz代理，Santa Cruz北京代理，Santa Cruz上海，Santa Cruz國內(nèi)代理
熱烈祝賀上海起發(fā)獲得Santa Cruz中國區(qū)代理 2013年9月開始正式簽約Santa Cruz，所有產(chǎn)品售后均有上海Santa Cruz負責(zé)，100%負責(zé)，并在上海備有20000多種現(xiàn)貨抗體，*，歡迎廣大經(jīng)銷商咨詢
http://m.duoo135.com/st178406/news_328698.html
上海起福生物科技有限公司Santa Cruz專業(yè)代理，具體產(chǎn)品信息歡迎電詢：
TECHNICAL SERVICE GUIDE: IMMUNOPRECIPITATION
Catalog #: Lot #:
A. Problem and Previous Experience
1. What is the specific problem you are experiencing?
2. Did this same vial of product work in the past? What were the results?
3. Did other lots of this product work in the past? Which lots? What were the results?
B. Samples
1. From which species （animal） was the sample and what type was used （cell lysate, tissue,
purified protein, etc.）? What lysis buffer was used?
2. How many cells were lysed or what was the protein concentration and volume of the sample that was used in the immunoprecipitation?
C. Pre-Clearing
1. Was a pre-clear step done prior to adding primary antibody?
2. What species of immunoglobulin （rabbit, goat, etc.） was used for the pre-clear step?
3. What protein agarose-conjugate was used in the pre-clear step （Protein-A, Protein-G Agarose, etc.）?
D. Antibodies and Immunoprecipitation Reagents
1. How many micrograms of primary antibody were added to the total cell lysate?
2. Was the primary antibody directly conjugated to agarose?
3. How long was the primary incubated with the total cell lysate?
4. What immunoprecipitation reagent was used （Protein-A Agarose, Protein-G Agarose, etc.）?
5. At what temperature has the primary antibody been stored? Has it ever been frozen?
E. Washes and Spins
1. Is the final wash done with a stringent reagent （RIPA） or a mild reagent （PBS）?
2. How fast were the samples spun （in RPM）?
上海起福生物科技有限公司
：楊建輝
：
辦公：
*8006
:
:http://www.qfbio.com/
地址：上海市浦東新區(qū)繡川路561號1102室
