国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊(cè)

當(dāng)前位置:
上海恒遠(yuǎn)生物科技有限公司>技術(shù)文章>恒遠(yuǎn)產(chǎn)品文獻(xiàn):試劑霍亂毒素引用文獻(xiàn)

技術(shù)文章

恒遠(yuǎn)產(chǎn)品文獻(xiàn):試劑霍亂毒素引用文獻(xiàn)

閱讀:871          發(fā)布時(shí)間:2020-12-29

【文獻(xiàn)標(biāo)題】Sphk1 participates in malignant progression of breast cancer by regulating epithelial-mesenchymal transition and stem cell characteristics

【作者】Zhijie Chen, Bingxiong Liu

【作者單位】漢川市人民醫(yī)院(Hanchuan People’s Hospital)

【文獻(xiàn)中引用產(chǎn)品】

霍亂毒素 CAS號(hào):9012-63-9

【關(guān)鍵詞】Breast cancer,Sphingosine kinase 1,Epithelial-mesenchymal transition,Stem cell,Metastasis

【DOI】doi.org/10.1016/j.tice.2020.101380

【影響因子(IF)】2.90

出版期刊】《Tissue and Cell》

【產(chǎn)品原文引用】

Cell and culture

Human breast epithelial cells (MCF-10A, CRL-10317) and BC cell lines MCF-7 (CRL-3435), T47D (HTB-133), SKBR3 (HTB-30), MDA-MB-231 (HTB-26), BT-474 (HTB-20) were purchased from American Tissue Culture Collection. The cells, except MDA-MB-23 cells, were cultured in DMEM (12491015, ThermoFisher, USA) containing 10 % FBS (30067334, ThermoFisher, USA), 1% penicillin/streptomycin (15140163,ThermoFisher, USA) at 37 °C in 5% CO2. MCF-10A cell line was cultured with additional 100 ng/mL cholera toxin (Hengyuan,China,hyswbio) on the basis of other cell culture condition. After culture for one day, the original medium was discarded, and the cells were washed 1–2 times by PBS. Next, 5 mL fresh DMEM medium was added and further incubated with the cells in a CO2 incubator. When the confluence reached 80%–90%, the cells were subcultured. MDA-MB-231 cells were cultured in Leibovitz’s L15 medium (11415064, ThermoFisher, USA) without CO2。

完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)!

 

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

對(duì)比框

產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 聯(lián)系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機(jī)商鋪
在線留言
仙游县| 朝阳县| 凌源市| 金沙县| 图木舒克市| 钦州市| 麻江县| 蒙山县| 余干县| 大石桥市| 成安县| 冀州市| 江孜县| 巨野县| 麦盖提县| 九江市| 娱乐| 四平市| 栾川县| 湄潭县| 应用必备| 南召县| 夏邑县| 辰溪县| 郁南县| 海盐县| 望江县| 神农架林区| 云龙县| 大英县| 房产| 方正县| 江山市| 凌海市| 延边| 梁平县| 彰武县| 自治县| 汶上县| 棋牌| 临海市|