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5-Methylcytidine-5'-Triphosphate代理

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更新時間:2016-12-27 19:22:50瀏覽次數(shù):2542次

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5-甲基胞苷-5-三磷酸鹽是TRILINK公司產(chǎn)品,用于干細(xì)胞研究用

5-甲基胞苷-5-三磷酸鹽是TRILINK公司新產(chǎn)品,用于干細(xì)胞研究用,5-methyl-ctp

Synonyms]
5-methyl-dCTP 
5-methyldeoxycytidine triphosphate 
CPD-1094
5-methyl deoxycytidine-5'-triphosphate 
5-methyl-2'-deoxycytidine-5'-triphosphate
cytidine 5'-(tetrahydrogen triphosphate), 2'-deoxy-5-methyl-
[[[5-[(4-amino-5-methyl-2-oxo-1H-pyrimidin-1-yl)]-3-hydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphinoyl]oxy-hydroxy-phosphinoyl]oxyphosphonic acid

[Structure]
 

5-methyl-dCTP ,5-methyldeoxycytidine triphosphate ,CPD-1094,5-methyl d


[ Properties Computed from Structure] 
 

Molecular Weight481.183503 [g/mol]
Molecular FormulaC10H18N3O13P3
XLogP-5.9
H-Bond Donor6
H-Bond Acceptor14
Rotatable Bond Count8
Tautomer Count3
Exact Mass481.005247
MonoIsotopic Mass481.005247
Topological Polar Surface Area248
Heavy Atom Count29
Formal Charge0
Complexity868
Isotope Atom Count0
Defined Atom StereoCenter Count0
Undefined Atom StereoCenter Count3
Defined Bond StereoCenter Count0
Undefined Bond StereoCenter Count0
Covalently-Bonded Unit Count1

Description 
5-Methyl-dCTP is widely used for construction of cDNA libraries 
 
Incorporation of 5-Methyl-dCTP 
M-MuLV Reverse Transcriptase 
Klenow Fragment of DNA Polymerase I 
Sequenase DNA Polymerase 
(Taq Polymerase, Vent) * 
Incorporation of Hg-dCTP 
DNA Polymerase I 

References to 5-Methyl-dCTP 
Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584. 
Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 (3):681. 
Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research6:369. 
* Wong et al. (1991) PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 (5):1081. 
Reference to Hg-dCTP 
Banfalvi et al. (1995) Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 (5):445. 
 

 

N-1014-15-Methylcytidine-5'-TP1umole1317.5
N-1014-105-Methylcytidine-5'-TP10umoles10625
N-1014-55-Methylcytidine-5'-TP5umoles6205

 

Reference(s)
Kariko K, Muramatsu H, Ludwig J, Weissman D. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. (2011) Nucleic Acids Research.
     
Kormann M, Hasenpusch G, Aneja M, et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. (2011) Nature Biotechnology 29:154–157.
     
Anderson, B., Muramatsu, H., Nallagatla, S.R., Bevilacqua, P.C., Sansing, L.H., Weissman, D. & Kariko, K. Incorporation of pseudouridine into mRNA enhances translation by dimishing PKR activation (2010) Nucleic Acids Research, 38(17): 5884-5892.
     
Warren et al., Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA, Cell Stem Cell (2010), doi:10.1016/j.stem.2010.08.012.
     
Kariko K, Muramatsu H, Welsh F, et al. Incorporation of Pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. (2008) Molecular Therapy (16)11: 1833-1840.
     
Kariko, K., Buckstein, M., Ni, H. & Weissman, D. Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA (2005). Immunity, 23(2), 165-175.
     
Lefmann M, et al. Novel Mass Spectrometry-based tool for genotypic identification of mycobacteria. (2004) Journal of Clinical Microbiology, 42(1): 339-346.
     
Hartmer R, Storm N, Boecker S, Rodi CP, Hillenkamp F, Jurinke C, van den Boom D. RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. (2003) Nucleic Acids Res., 31(9): e47.
     
Nguyen A, Zhao C, Dorris D, Mazumder A. Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios. (2002) BMC Biotechnology, 2(1): 14.
     
Van Rompay AR, Norda A, Linden K, Johansson M, Karlsson A. Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. (2001) Mol Pharmacol., 59(5): 1181-6.
     


 

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