| I. Background | |
皰疹病毒進(jìn)入介質(zhì)（HVEM），也稱為腫瘤壞死因子受體超家族成員 14 （TNFRSF14），是 TNF 受體（腫瘤壞死因子）超家族的人細(xì)胞表面受體。HVEM 在多種 免疫細(xì)胞中表達(dá)，包括 T 細(xì)胞、B 細(xì)胞、自然殺傷細(xì)胞、樹突狀細(xì)胞和內(nèi)皮細(xì)胞。它還通 過(guò)在黑色素瘤細(xì)胞上的頻繁表達(dá)參與腫瘤的免疫逃避。 | |
| II. Description | |
HVEM NFκB-Luc Jurkat 報(bào)告基因藥靶模型很好的模擬了體內(nèi) HVEM 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程， 原理見下圖所示。 Figure 1. HVEM NFκB-Luc Jurkat 細(xì)胞模型原理圖 | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | HVEM-NFκB-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR, HVEM Cell line, HVEM NFAT |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800μg/ml hygromycin+1μg/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
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Figure 2. Recombinant Jurkat stably expressing human HVEM. | |
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). HVEM NFκB-Luc Jurkat(C7) Report Assay Stimulated by LIGHT/CHO.
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