目錄:愛必信（上海）生物科技有限公司>>細胞生物學(xué)>>重組蛋白>> abs00918Recombinant Human IFN-λ1/IL-29
| 供貨周期 | 一周 | 規(guī)格 | 5ug |
|---|
| 概述 | |
|---|---|
| 種屬 | Human |
| 形態(tài) | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | GPVPTSKPTT TGKGCHIGRF KSLSPQELAS FKKARDALEE SLKLKNWSCS SPVFPGNWDL RLLQVRERPV ALEAELALTL KVLEAAAGPA LEDVLDQPLH TLHHILSQLQ ACIQPQPTAG PRPRGRLHHW LHRLQEAPKK ESAGCLEASV TFNLFRLLTR DLKYVADGNL CLRTSTHPES T |
| 內(nèi)毒素水平 | Less than 1 EU/μg of Recombinant HumanIFN-λ1/IL-29 as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg. |
| Accession # | Q8IU54 |
| 預(yù)期分子量 | Approximay 19.8 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 97 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 282618 |
| 參考文獻 | |
|---|---|
| 1. Miller EK, Hernandez JZ, Wimmenauer V, et al. 2012. Am J Respir Crit Care Med, 185: 508-16.<br/>2. Li Y, Xie J, Xu X, et al. 2012. Protein Cell, <br/>3. Jordan WJ, Eskdale J, Srinivas S, et al. 2007. Genes Immun, 8: 254-61.<br/>4. Pekarek V, Srinivas S, Eskdale J, et al. 2007. Genes Immun, 8: 177-80.<br/>5. Megjugorac NJ, Gallagher GE, Gallagher G. 2010. Blood, 115: 4185-90. | |
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