Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~780 nm.

AAT Bioquest offers this preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

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別藻藍(lán)蛋白 (APC) 是一種從藍(lán)綠藻螺旋藻中分離出來(lái)的藻膽蛋白。與其他藻膽蛋白一樣，APC 是熒光的，具有J高的吸收率和高量子效率。它是一種可以通過(guò)常規(guī)蛋白質(zhì)交聯(lián)技術(shù)輕松與抗體和其他蛋白質(zhì)連接而不改變其光譜特性的蛋白質(zhì)。APC-Cy7 是流式細(xì)胞術(shù)中常用的顏色。其主要吸收峰在 651 nm，發(fā)射峰在 ~780 nm。

艾美捷預(yù)激活A(yù)PC-Cy7串聯(lián) 以促進(jìn) APC-Cy7 與抗體和其他蛋白質(zhì)（如鏈霉親和素和其他二級(jí)試劑）的串聯(lián)偶聯(lián)。該預(yù)激活A(yù)PC-Cy7串聯(lián)已準(zhǔn)備好進(jìn)行偶聯(lián)，與傳統(tǒng)繁瑣的基于 SMCC 的偶聯(lián)化學(xué)相比，其產(chǎn)量要高得多。

艾美捷預(yù)激活A(yù)PC-Cy7串聯(lián)普通儲(chǔ)備溶液制備：

將特定質(zhì)量的ReadiUse™ 預(yù)活化 APC-Cy7 Tandem復(fù)溶至給定濃度所需的適當(dāng)溶劑體積。請(qǐng)注意，體積僅用于制備儲(chǔ)備溶液。

艾美捷預(yù)激活A(yù)PC-Cy7串聯(lián)組件：

A：ReadiUse™ 預(yù)激活 APC-Cy7 串聯(lián) 1 瓶（1 毫克）

B：Buccutite™ MTA 1 瓶 (100 µg)

C：旋轉(zhuǎn)脫鹽柱 不包含

光譜特性：

消光系數(shù)（cm -1 M -1） 700000

激發(fā)（納米） 651

發(fā)射（納米） 779

儲(chǔ)存、安全和處理：

H-短語(yǔ) H303、H313、H333

危險(xiǎn)符號(hào) XN

有可能的使用 僅供研究使用 (RUO)

R-短語(yǔ) R20、R21、R22

UNSPSC 12171501