艾美捷PolyMag CRISPR試劑盒#PNC40200高轉(zhuǎn)染效率無毒性，靈敏性高、特異性強，用途范圍廣，具體特色如下：

• 即使在原代細胞和難以轉(zhuǎn)染的細胞上也能高效遞送 CRISPR/Cas9 系統(tǒng)• 非常適合共轉(zhuǎn)染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA

• 針對含有 Cas9 的大質(zhì)粒 DNA 進行了優(yōu)化，并引導(dǎo) RNA

• 低細胞毒性 - 能夠以更少的細胞開始實驗

PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection™ technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.

Benefits:

•    High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells

•    Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA

•    Optimized for large plasmid DNA containing Cas9, and guide RNA

•    Low cell toxicity - enable to start your experiment with less cell

用于CRISPR Cas9實驗的其他產(chǎn)品：

ViroMag CRISPR提高CRISPR/Cas9病毒的轉(zhuǎn)導(dǎo)效率

RmesFect CRISPR 用于 mRNA 轉(zhuǎn)染

ProDeliverIN CRISPR用于 Cas9 蛋白遞送

儲存： +4°C

運輸條件：室溫

Figure1 : PolyMag CRISPR co-transfection efficiency for CRISPR-Cas9 experiments. NIH-3t3, HeLa and MDCK cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transfection. Cells were transfected with a GFP-Cas9 plasmid and a RFP pDNA and then incubated overnight at 37oC. GFP and RFP expression was monitored 24h after transfection by fluorescence microscopy.