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大鼠膠質(zhì)肉瘤細(xì)胞9L/lacZ

	大鼠
別稱	9L/LacZ
組織來源	腦，膠質(zhì)細(xì)胞
疾病	膠質(zhì)肉瘤
傳代比例/細(xì)胞消化	1:2傳代，消化1-2分鐘
培養(yǎng)基配置	DMEM培養(yǎng)基；10%胎牛血清；1%雙抗
簡介	9L/lacZ細(xì)胞株1989年從9L細(xì)胞株（大鼠xj誘導(dǎo)的膠質(zhì)瘤細(xì)胞株）發(fā)展而來。用攜帶E. coli編碼beta-半乳糖苷酶lacZ基因和帶來G418抗性的Tn5基因BAG復(fù)制缺陷的逆轉(zhuǎn)錄病毒載體感染9L細(xì)胞株。細(xì)胞在G418存在下培養(yǎng)14天，克隆，并檢測beta-半乳糖苷酶生成。 9L/lacZ產(chǎn)生高水平的酶，選擇其進(jìn)行后續(xù)研究。細(xì)胞持續(xù)表達(dá) lacZ報(bào)告基因產(chǎn)物，從E. coli衍生來的beta-半乳糖苷酶，從而可以通過組織切片的組織化學(xué)染色來鑒定單個(gè)腫瘤細(xì) 胞。同一片子上的淋巴細(xì)胞和其它響應(yīng)細(xì)胞也可以通過雙標(biāo)記抗體進(jìn)行鑒定。染色細(xì)胞和背景的對(duì)比有益于圖像分析。這是少數(shù)允許量化分析的大腦微觀腫瘤模型中的一種。這種腫瘤模仿了人類大腦腫瘤的生長和傳播的重要特性。 beta-半乳糖苷酶的表達(dá)十分穩(wěn)定，但細(xì)胞培養(yǎng)數(shù)月后應(yīng)該重新進(jìn)行克隆。
形態(tài)	成纖維細(xì)胞樣
生長特征	貼壁生長
倍增時(shí)間	每周 2 至 3 次
基因表達(dá)	beta galactosidase (beta-gal)
致瘤性	Yes, forms tumors in the brains of CD Fischer 344 rats
保藏機(jī)構(gòu)	ATCC; CRL-2200

The authors declare that they have no known cogpeting financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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