An NF-κB luciferase reporter construct is stably integrated into the genome of CHO-K1 cells. The firefly luciferase gene is controlled by the NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

The NF-κB-luciferase / CHO-K1 cell line is suitable for monitoring the activity of NF-κB
transcription factor through luminescence readout.). This cell line responds to human cytokine
IL-1β, responds moderately to human TNF, and does not respond to human IFN-λ (2 μg/ml).
Reducing the amount of serum during incubation period may increase the sensitivity to
cytokines. Since CHO-K1 cells do not express endogenous human proteins, this cell line
provides an excellent platform to enable exogenous expression of a protein of interest to study
its downstream effect on NF-κB signaling.

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