The Mouse PDE10A Assay Kit is designed for identification of inhibitors of Mouse PDE10A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE10A to the binding agent.

The key to the Mouse PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE10A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE10A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.