The PARP3 Chemiluminescent Assay Kit is designed to measure PARP3 activity for screening and profiling applications. The PARP3 assay kit 96-well or 384-well format contains enough purified PARP3 enzyme, histone mixture, activated DNA template, PARP assay buffer and necessary reagents for 100 or 400 enzyme reactions.

Figure 1. PARP3 Chemiluminescent Assay Kit schematic.
The key to the PARP3 Chemiluminescent Assay Kit is the biotinylated NAD⁺ and involves 3 reactions. First, histone proteins are coated on a plate. Next, a biotinylated NAD⁺ mix (termed PARP Substrate Mixture) is incubated with the enzyme and an activated DNA template. Finally, streptavidin-HRP is added, followed by ELISA ECL substrate to produce a chemiluminescence signal that can be measured using a chemiluminescence plate reader.

*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

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