The PARP3 Homogeneous Assay Kit is designed to measure PARP3 activity for screening and profiling applications. PARP3 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP3 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, a sample containing PARP3 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Figure 1: PARP3 Homogenous Assay Kit schematic