The PARP11 Homogeneous Assay Kit is designed to measure PARP11 activity for screening and profiling applications. PARP11 is known to catalyze the NAD-dependent addition of ADP-ribose to its substrates. The key to the PARP11 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP11 reactions. First, a sample containing PARP11 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Figure 1: PARP11 Homogenous Assay Kit schematic