The Quick PCR^TM Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit does not include E. coli cells; however, another version of the kit includes E.coli chemically competent cells for transformation (BPS Bioscience #78532).