The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity for screening and profiling applications. The NSD3 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified NSD3 enzyme for 96 enzyme reactions. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.