The JMJD2A Homogeneous Assay Kit is designed to measure activity of the JMJD2A for screening and profiling applications, using AlphaLISA^® technology. The key to the JMJD2A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

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