The DCAF15 Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) Assay Kit designed to measure DCAF15 (DDB1 and CUL4-associated factor 15) auto-ubiquitination activity in a homogeneous 384-reaction format. It utilizes Europium-labeled Ubiquitin (donor) and Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF15. As a homogenous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses.

Figure 1. DCAF15 Intrachain TR-FRET Assay Kit schematic