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BPS Bioscience Inc.

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Ubiquitination

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Description

The C-CBL-driven AXL Ubiquitination Intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination.

Figure 1: C-CBL-driven AXL ubiquitination intrachain TR-FRET Assay Kit schematic.

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Synonyms

E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, Signal transduction protein CBL, CBL, CBL2, RNF55, UFO, AXL oncogene, Tyrosine-protein kinase

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