国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊(cè)

當(dāng)前位置:
美國(guó)布魯克海文儀器公司>技術(shù)文章>90Plus Zeta測(cè)量應(yīng)用案例-2016-15

技術(shù)文章

90Plus Zeta測(cè)量應(yīng)用案例-2016-15

閱讀:346          發(fā)布時(shí)間:2016-5-26

文獻(xiàn)名: A study of the synergistic effect of folate-decorated polymeric micelles incorporating Hydroxycamptothecin with radiotherapy on xenografted human cervical carcinoma

作者: Hong Youa, ShaoZhi Fua, XingHu Qinb, YanXin Yua, Bo Yanga, GuangPeng Zhanga, XiaoYang Suna, Yue Fengc, Yue Chenc, JingBo Wua
a Department of Oncology, the First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
b Department of Neurosurgery, the First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
c Department of Nuclear Medicine, the First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China


摘要:In this study, Hydroxycamptothecin (HCPT)-loaded micelles were formed in water by the self-assembly of folate (FA)-decorated amphiphilic block copolymer, methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL), and achieved a hydrodynamic diameter about of 132 nm. HCPT release from the micelles exhibited no initial burst but showed a sustained release profile. The cytotoxicity and targeting ability of FA conjugated polymeric micelles was investigated by using methylthiazoletetrazolium (MTT) and fluorescence microscopy. We found that FA-conjugated micelles had superior cytotoxicity against HeLa cells compared to non-conjugated micelles, and that they exerted this effect by folate receptor (FR)-mediated endocytosis. In addition, HeLa cells were xenografted into nude mice and subjected to radiotherapy (RT) and/or HCPT-loaded micelle treatment. The antitumor efficacy was detected by analysis of tumor growth delay (TGD) and median survival time. Micro fluorine-18-deoxyglucose PET/computed tomography (18F-FDG PET/CT) was performed to assess early tumor response to HCPT-loaded micelles in combination with RT. Analysis of cell cycle redistribution, apoptosis and expression of histone H2AX phosphorylation (λ-H2AX) was used to evaluate the mechanism by which HCPT loaded micelles led to radiosensitization. Taken together, the results showed that HCPT-loaded FA decorated micelles efficiently sensitized xenografts in mice to RT, and induced G2/M phase arrest, apoptosis and expression of λ-H2AX.

關(guān)鍵詞:Radiotherapy; 10-Hydroxycamptothecin; Micelle; Folate; Targeted drug delivery; Synergistic effect; Mechanism
 

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

對(duì)比框

產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 聯(lián)系電話 二維碼 意見(jiàn)反饋 在線交流

掃一掃訪問(wèn)手機(jī)商鋪
010-62081908
在線留言
黄浦区| 璧山县| 阳新县| 太湖县| 宁化县| 武安市| 新竹市| 谢通门县| 娄底市| 赤城县| 巴塘县| 庆安县| 修文县| 云林县| 安徽省| 虞城县| 安多县| 班玛县| 伊宁县| 奉贤区| 万荣县| 苏州市| 秭归县| 犍为县| 昂仁县| 鹤庆县| 蓝山县| 梁河县| 夏河县| 合水县| 大邑县| 武夷山市| 苍山县| 长治县| 昭通市| 富阳市| 河北省| 韶关市| 会宁县| 郯城县| 旬邑县|