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Attachment from Flow of Escherichia coli Bacteria onto Silanized Glass Substrates
閱讀：257 發(fā)布時(shí)間：2015-4-13| 提 供 商 | 美國(guó)布魯克海文儀器公司 | 資料大小 | 1.8MB |
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We investigate the attachment of Escherichia coli
on silanized glass surfaces during flow through a linear channel
at flow rates of 0.11 mL/min using confocal microscopy. We
assemble layers of organosilanes on glass and track the position
and orientation of bacteria deposited on these surfaces during
flow with high spatial resolution. We find that a metric based on
the degree of the surface-tethered motion of bacteria driven by
flagella is inversely correlated with deposition rate, whereas
conventional surface characterizations, such as surface energy or
water contact angle, are uncorrelated. Furthermore, the
likelihood that an initially moving bacterium becomes immobilized increases with increasing deposition rate. Our results
suggest that the chemistry and arrangement of silane molecules on the surface influence the transition from transient to
irreversible attachment by favoring different mechanisms used by bacteria to attach to surfaces.