Activation of tumor suppressor p53 gene expression by magnetic thymine-imprinted chitosan nanoparticles

作者： Mei-Hwa Lee,a   James L. Thomas,b   Jian-Zhou Chen,c   Jeng-Shiung Jand and    Hung-Yin Lin*c

a

Department of Materials Science and Engineering, I-Shou University, Kaohsiung 840, Taiwan

b

Department of Physics and Astronomy, University of New Mexico, Albuquerque, USA

c

Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan

: linhy@ntu.edu.tw

d

Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan

摘要：Chitosan is a natural biodegradable polysaccharide that has been used to enhance gene delivery, owing to the ease with which chitosan nanoparticles enter the nucleus of cells. To study the effects of nuclear delivery of omeric gene sequences, which contain thymine, we formed magnetic thymine-imprinted chitosan nanoparticles (TIPs) by the precipitation of chitosan, mixed with thymine and magnetic nanoparticles (to aid in separations). The mean size of the TIPS was 116 ± 18 nm; the dissociation constant for thymine was 21.8 mg mL−1. We then treated human hepatocellular carcinoma (HepG2) with TIPs nanoparticles bearing bound thymine or a bound omeric DNA sequence. The expression of the tumor suppressor p53 gene increased when TIPs were applied and decreased when omere-bound TIPs were applied.