Organism:

Homo sapiens, human

Tissue:

peripheral blood

Culture Properties:

suspension

Morphology:

lymphoblast

Biosafety Level:

2

Complete Growth Medium:

RPMI-1640(Gibco31800-022)+10%FBS+ Penicillin/Streptomycin

Culture Conditions:

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Subculturing:

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1×10⁵ viable cells/mL. Do not allow cell concentration to exceed 1×10⁶ cells/mL.

Interval: Maintain cell density between 1×10⁵ and 1×10⁶ viable cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation:

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase