invitrogen/A11029/A11034二抗*

訂貨找螞蟻淘就對(duì)了，4000-520-626

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11034) in IF

Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate (Product # A-11034) was used at a concentration of 4 µg/ml in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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螞蟻淘常見(jiàn)平行進(jìn)口產(chǎn)品有：
Thermofisher旗下Gibco培養(yǎng)系列、ABI細(xì)胞計(jì)數(shù)儀及PCR儀、Invitrogen分子生物學(xué)試劑及Qubit3.0、Ambion的RNA相關(guān)試劑、Molecular probes試劑、Pierce蛋白相關(guān)試劑盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich試劑；AccuStandard 標(biāo)準(zhǔn)品；Millipore抗體；Glen Research 核酸合成試劑；Bio-Rad電泳儀及PCR儀；Nanocs的PEG聚合物；  Chromotek羊駝抗體；   Polysciences轉(zhuǎn)染試劑；  Echelon-inc 脂類研究試劑盒；   R&D Systems抗體及Elisa試劑盒； Jackson二抗及封閉血清； Biotium 熒光染料；NEB內(nèi)切酶及NGS相關(guān)產(chǎn)品；  Athens Research & Technology人類蛋白及抗血清；  Illumina二代測(cè)試試劑盒； Tocris 小分子試劑； TwistDx恒溫?cái)U(kuò)增試劑盒（RPA）； Enzymatics二代測(cè)序酶；Novus抗體及Elisa試劑盒；AG scientific潮霉素B、G-418；Plantmedia植物培養(yǎng)系列及抗體；Agilent 2100生物分析儀；Biolog耗材；Biomatik的Elisa試劑盒；Covaris基因打斷儀；CST抗體；Dharmacon的siRNA產(chǎn)品。