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備注：本公司銷售的所有產(chǎn)品僅用于生化科研試驗

纖維粘連蛋白（FN）存在于間隙基質(zhì)和血漿中，通過RGD序列介導(dǎo)細(xì)胞的粘附。FN的主要功能是在傷口愈合過程中促進(jìn)細(xì)胞的遷移。有FN包被的細(xì)胞培養(yǎng)表面，對細(xì)胞的粘附，伸展和增殖有促進(jìn)作用。FN也可以用作無血清培養(yǎng)基的添加物。

來源：人血漿
分子量：440 kD
使用指南：根據(jù)細(xì)胞類型不同，推薦工作濃度為1-5 μg/cm²生長面積或5 ug/ml培養(yǎng)添加劑。
保存條件和效期：凍干粉2-8℃下保存或溶解后-20℃下保存，避免多次凍融。保質(zhì)期詳見產(chǎn)品包裝。



 

Description

Fibronectin, human, used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types.

Quality

Purity ≥ 90% by SDS-PAGE
Tested for ability to promote attachment and spreading of BHK-21 cells
Filtered （0.2 µm membrane） and tested and found negative for bacteria, fungi, and mycoplasma

Source

Human plasma. NOTE: Source material tested for hepatitis B antigen and HIV-1 antibody.

Molecular Weight

440 kD （unreduced form）

Preparation and Storage

Stable for three months at 2° to 8°C （lyophilized） or two weeks at -20°C （solubilized）. Do not store in frost-free freezer. Avoid multiple freeze-thaws

Handling

Instructions for Use
Recommended concentration - 1-5 µg/cm2 of growth surface for coating or at 5 µg/ml as a media additive （guidelines for coating included with product）.

Formulation

Lyophilized from CAPS buffer containing NaCl and CaCl2, pH 11.0; reconstitute in dH20 （do not agitate or swirl）.

Product Details

Catalog Number

354008

Brand

BD™

Name

Fibronectin, human, 1 mg

Size

1 mg

Regulatory Status

 RUO

Labware details - Contents and Storage

Type Full:

Fibronectin, human

Quantity Pack:

0 type :

Quantity Case:

0

Packaging:

1 mg

Shipping:

Refrigerated shipping WI

Storage:

Storage: 2-8°C （lyophilized） or two weeks at -20°C （solubilized）; Shelf life: >= 3 months from date of shipment