以75 mmol/L的二氧化氯作用于脫氧核糖核苷三磷酸dCTP、dTTP、dGTP、dATP混合物(濃度均為100 mmol/L), 液相色譜顯示它們?cè)?60 nm波長處的峰面積分別下降了54.23%±2.08%、66.25%±3.32%、55.47%±0.23%、59.59%±3.27%。純化質(zhì)粒被二氧化氯作用后電泳條帶未出現(xiàn)明顯彌散和拖尾現(xiàn)象。45 mmol/L以上二氧化氯能*抑制質(zhì)粒DNA的PCR模板活性, 150 mmol/L的二氧化氯才能使純化質(zhì)粒的轉(zhuǎn)化率降至對(duì)照組的0.20%±0.20%。實(shí)驗(yàn)證明二氧化氯對(duì)DNA具有損傷作用, 這種損傷可能與嘧啶堿和嘌呤堿的共軛雙鍵被破壞有關(guān)。

Chlorine dioxide, Plasmid, HPLC, Template, Transformation rate

The mixture of deoxyribonucleoside, dCTP, dTTP, dGTP and dATP at concentration of 100 μmol/L for each, was treated by 75 mmol/L of chlorine dioxide for 10 min, high performance liquid chromatographic analyses indicated that all of the four decreased in peak area by 54.23%±2.08%, 66.25%±3.32%, 55.47%±0.23%, 59.59%±3.27% respectively at wavelength of 260 nm. For 0.2 μg/μL of purified plasmid DNA, their activity as template in polymerase chain reaction were completely inhibited by chlorine dioxide at concentration of 45 mmol/L or above, and the transformation rate decreased to 0.20%±0.2% when treated by 75 mmol/L of chlorine dioxide. Our result indicated that chlorine dioxide have substantial damages on deoxyribonucleoside and plasmid DNA.