017-000-121正常驢血清英文說明:
Information
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Usage
Physical State: Freeze-dried solid
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
017-000-121正常驢血清(艾美捷)中文說明:
正常血清經(jīng)脂質(zhì)提取以提高透明度,用含疊丨氮丨化鈉的磷酸鹽緩沖鹽水(PBS)透析,然后冷凍干燥。強烈推薦在PBS中稀釋至5%(v/v)的正常血清作為阻斷試劑,以減少非特異性、保守序列和/或Fc受體結(jié)合的背景。在添加一級抗體之前,使用來自與標(biāo)記抗體相同的宿主的稀釋的正常血清作為單獨的孵育步驟獲得最佳結(jié)果。
艾美捷017-000-121正常驢血清基本參數(shù):
Host: Donkey
Product:
Conjugate: Unconjugated
Product Category: Normal Serums and Gamma Globulins
RRID: AB_2337258
注意:
1、017-000-121正常驢血清可應(yīng)用于大多數(shù)免疫學(xué)實驗,用作封閉液或空白對照.
2、凍干粉末形式,保存在2-8°C,準(zhǔn)備用時,根據(jù)指示的體積加入雙蒸水,在2-8°C條件下,未稀釋的液體保質(zhì)期6周,每天準(zhǔn)備新鮮的工作液.如果重溶后,想延長保存期,將未稀釋的溶液保存在-20°C或者更低,避免反復(fù)凍融.
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