115-035-003 HRP標(biāo)記親和純化山羊抗小鼠IgG（H+L）英文說明：

Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)

Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)

Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry

1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates

1:10,000 - 1:200,000 for Western blotting with ECL substrates

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

115-035-003 HRP標(biāo)記親和純化山羊抗小鼠IgG（H+L）（艾美捷）中文說明：

基于免疫電泳和/或ELISA，抗體與全分子小鼠IgG反應(yīng)。它還與其他小鼠免疫球蛋白的輕鏈反應(yīng)。未檢測(cè)到針對(duì)非免疫球蛋白血清蛋白的抗體。該抗體可能與其他物種的免疫球蛋白發(fā)生交叉反應(yīng)。

通過免疫親和層析從抗血清中分離出完整的IgG抗體。它們具有通過二硫鍵連接在一起的Fc部分和兩個(gè)抗原結(jié)合Fab部分，因此它們是二價(jià)的。據(jù)報(bào)道，平均分子量約為160kDa。整個(gè)IgG形式的抗體適用于大多數(shù)免疫檢測(cè)程序。

115-035-003辣根過氧化物酶HRP標(biāo)記親和純化山羊抗小鼠IgG（H+L）基本參數(shù)：

目標(biāo)：鼠

宿主：山羊

抗體形式：全IgG

特異性：IgG（H+L）

標(biāo)記物：辣根過氧化物酶

類別：全IgG親和純化抗體

克隆性：多克隆

物理狀態(tài)：凍干固體

儲(chǔ)存和再水合：在2-8°C下儲(chǔ)存凍干固體。用指-定體積的dH2O重新水合，如果不清楚，則進(jìn)行離心。使用當(dāng)天準(zhǔn)備工作稀釋液。在2-8°C下作為未稀釋液體穩(wěn)定約6周。

脫水后的長(zhǎng)期儲(chǔ)存：等分并在-70°C或更低溫度下冷凍。避免反復(fù)冷凍和解凍。或者，添加等量的甘油（ACS級(jí)或更好），最終濃度為50%，并以液體形式儲(chǔ)存在-20°C。

純度：抗體通過免疫親和層析從抗血清中純化，使用偶聯(lián)到瓊脂糖珠的抗原。

緩沖液：0.01MNa₃PO?，0.25M NaCl，pH 7.6

穩(wěn)定劑：15 mg/ml牛血清白蛋白（無IgG，無蛋白酶）

防腐劑：無

建議工作濃度或稀釋范圍：

1： 500-1:5000用于免疫組織/細(xì)胞化學(xué)

1： 5000-1:100000用于ELISA和顯色底物的蛋白質(zhì)印跡

1： 10000-1:200000用于ECL底物的蛋白質(zhì)印跡

稀釋因子以范圍的形式表示，因?yàn)?/span>合適的稀釋是許多因素的函數(shù)，如抗原密度、滲透性等。實(shí)際使用的稀釋必須根據(jù)經(jīng)驗(yàn)確定。