Norgen's NGS Normalization 96-Well Kit allows for high-throughput PCR amplicon clean-up and normalization of PCR product concentration (~5 ng/µL). The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized PCR amplicon can then be used in NGS workflows and other sequencing applications.

For high-throughput NGS Library PCR amplicon clean-up and normalization of NGS library PCR product concentration

NGS Normalization 96-Well Kit：

√Removes primer dimers

√Simultaneously clean-up and normalize PCR products

√Fast (less than 20 minutes), high-throughput and easy processing using centrifugation

√Sufficient elution volume (100 µL) for repeat or future assays

√Non-magnetic bead purification

艾美捷高通量NGS標(biāo)準(zhǔn)化96孔板試劑盒#NGB-61900：

中文名稱：NGS標(biāo)準(zhǔn)化96孔板試劑盒

英文名字：NGS Normalization 96-Well Kit

Norgen Biotek編號(hào)：NGB-61900

規(guī)格：2Plates

保存建議：溶液密封室溫(15-25°C)保存，未開(kāi)封至少保存1年。

NGS標(biāo)準(zhǔn)化96孔試劑盒組件包含：

組件 NGS Normalization

Buffer SK 30 mL

Wash Solution A 2x 38 mL

Elution Buffer B 30 mL

Adhesive Tape 1

96-Well Normalization Plate 2

96-Well Collection Plate 2

96-Well Elution Plate 2

Product Insert 1

NGS標(biāo)準(zhǔn)化96孔試劑盒（NGS Normalization）儲(chǔ)存條件所有溶液均應(yīng)密封并在室溫下儲(chǔ)存。 本試劑盒在裝運(yùn)日期后2年內(nèi)保持穩(wěn)定。

高通量NGS標(biāo)準(zhǔn)化96孔板試劑盒特色：

√去除引物二聚體

√同時(shí)凈化和標(biāo)準(zhǔn)化PCR產(chǎn)物

√快速（不到 20 分鐘）、高通量且易于離心處理

√足夠的洗脫體積 （100 μL），用于重復(fù)或?qū)?lái)的分析

√非磁珠純化