VectaPlex™ Antibody Removal Kit (VRK-1000)
Q: VectaPlex™可用于冰凍切片嗎?
A: No. During development, VectaPlex™ was optimized for FFPE tissues, where the power to remove antibodies at room temperature was balanced with the need to preserve tissue morphology. For frozen tissues, VectaPlex™ will remove the tissue from the slide.
Q: VectaPlex™ 可以去除免疫組化 (IHC) 實驗應用中的抗體染色(酶顯色)嗎?
A: VectaPlex™ Antibody Removal Kit is designed to be used in fluorescent workflows such as immunofluorescence or TSA. It has not been validated or confirmed for immunohistochemistry (IHC) applications. VectaPlex™ may remove substrates already deposited on tissue samples.
Q: 我有使用TrueView試劑盒去除組織的自發(fā)熒光, 請問TrueVIEW會被VectaPlex™試劑去除嗎?
A: We used TrueView in our VectaPlex™ IF workflow to suppress autofluorescence. Application of VectaPlex™ removes some, but not all, of the TrueView reagent. Since some TrueView remained after VectaPlex™ application, we did not add TrueView in every cycle, but only as needed to suppress autofluorescence.
Q: 請問將VectaPlex™試劑盒放置到4℃冰箱一段時間,還可以使用嗎?
A: Yes. VectaPlex™can withstand a freeze-thaw cycle and still perform as expected, but the recommended storage is at ambient temperature (15-25 °C). Additionally, the kit will still perform properly if placed at 4 °C.
Q: 在同一張切片上使用 VectaPlex™,是否有最大次數(shù)限制呢?
A: VectaPlex™ has been tested for up to 6 cycles with no ill effects. We don’t expect there to be issues with increasing the number of cycles, but we suggest testing with controls for adequate comparisons. We recommend running experiments to confirm that the use of more cycles does not impact the antigenicity or tissue morphology.
Q: VectaPlex™可用于自動化染色平臺嗎?
A: We have not tested this product on automated systems and cannot guarantee instrument compatibility at this time.
Q: VectaPlex™ 是否適用于所有種屬來源的抗體?
A: VectaPlex™ has been tested with mouse and rabbit primaries, and with horse and goat secondaries. We expect it to work well with other species of antibodies as well.
Q: VectaPlex™ 是否適用于不同類型的組織樣本?
A: VectaPlex™ has been tested on and works well with various FFPE tissues, including FFPE breast and lung tissue which are very fragile.
Q: VectaPlex™ 是否與所有抗體相兼容?
A: VectaPlex™ has been tested on more than 30 common antibodies and has worked on them without issue.
Q: 在IF實驗中, VectaPlex™ 抗體去除試劑是否也可以去除組織的自發(fā)熒光呢?
A: VectaPlex™ does not appear to reduce autofluorescence background.
Q: 是否可以提供使用VectaPlex™操作過程中移去蓋玻片的操作流程呢?
A: We recommend using VectaShield® Plus Antifade Mounting Media with DAPI (H-2000), which is an aqueous non-hardening mounting medium. After each imaging cycle, the coverslip can be easily removed by incubating in PBS and the residual VectaShield® removed with a PBS wash.
詳情請聯(lián)系 Vectorlabs總代理商 -欣博盛生物科技
相關產(chǎn)品
免責聲明
- 凡本網(wǎng)注明“來源:化工儀器網(wǎng)”的所有作品,均為浙江興旺寶明通網(wǎng)絡有限公司-化工儀器網(wǎng)合法擁有版權或有權使用的作品,未經(jīng)本網(wǎng)授權不得轉載、摘編或利用其它方式使用上述作品。已經(jīng)本網(wǎng)授權使用作品的,應在授權范圍內使用,并注明“來源:化工儀器網(wǎng)”。違反上述聲明者,本網(wǎng)將追究其相關法律責任。
- 本網(wǎng)轉載并注明自其他來源(非化工儀器網(wǎng))的作品,目的在于傳遞更多信息,并不代表本網(wǎng)贊同其觀點和對其真實性負責,不承擔此類作品侵權行為的直接責任及連帶責任。其他媒體、網(wǎng)站或個人從本網(wǎng)轉載時,必須保留本網(wǎng)注明的作品第一來源,并自負版權等法律責任。
- 如涉及作品內容、版權等問題,請在作品發(fā)表之日起一周內與本網(wǎng)聯(lián)系,否則視為放棄相關權利。