產(chǎn)品名稱    THLE-2（人肝永生化細胞）

別稱    THLE2;Transformed Human Liver Epithelial-2

種屬    人

生長特性    貼壁細胞

細胞形態(tài)    上皮細胞樣

凍存條件    凍存液：55% DM/F12+40% FBS+5% DMSO

溫度：液氮

培養(yǎng)方案A（默認(rèn)）    生長培養(yǎng)基：THLE-2細胞專用培養(yǎng)基

培養(yǎng)條件：氣相：空氣，95%；CO?，5%, 溫度：37℃

推薦傳代比例    1:3-1:6

推薦換液頻率    2-3次/周

參考資料(來源文獻)

背景描述    THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways.

年齡（性別）    Adult;Male

組織來源    Liver; Left lobe

細胞類型    轉(zhuǎn)化細胞系

生物安全等級    BSL-2

致瘤性    No; No, nude mice.

保藏機構(gòu)    ATCC; CRL-2706