Designations:

Biosafety

Level:

Medium

Serum:

Organism:

Source:

Homo sapiens (human)

Organ: thyroid

Disease: papilloma

Morphology:

Growth

Properties:

Propagation:

epithelial

adherent

& See Propagation

TPC-1

1

細(xì)胞庫

The base medium for this cell line is formulated RPMI-1640 Medium. To make the complete

growth medium, add the following components to the base medium: fetal bovine serum to

a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:

Protocol:

1. Remove and discard culture medium.

2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to

remove all traces of serum that contains trypsin inhibitor.

3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an

inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while

waiting for the cells to detach. Cells that are difficult to detach may be placed at

37°C to facilitate dispersal.

4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5. Add appropriate aliquots of the cell suspension to new culture vessels.

6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Preservation:

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase