Product nameProlactin Human SimpleStep ELISA? Kit See all Prolactin kitsDetection methodColorimetricPrecision Inter-assay Sample n Mean SD CV% Human milk 3 9.9% Sample typeCell culture supernatant, Milk, Serum, Heparin Plasma, EDTA PlasmaAssay typeSandwich (quantitative)Sensitivity 70 pg/mlRange0.39 ng/ml - 50 ng/mlRecovery Sample specific recovery Sample type Average % Range Milk 117.1 111.7% - 121% Serum 92.1 89.9% - 93.2% Cell culture media 96.3 94.2% - 99% Heparin Plasma 95.9 92.1% - 97% EDTA Plasma 95.7 92.1% - 97.5% Assay time1h 30m Assay durationOne step assaySpecies reactivityReacts with: Human Product overview Abcam’s Prolactin in vitro SimpleStep ELISA? (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prolactin protein in Human serum, plasma, milk and cell culture supernatant samples. The SimpleStep ELISA? employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. Sensitivity: Samples diluted in Sample Diluent 25BS - 110 pg/mL Samples diluted in Sample Diluent NS – 70 pg/mL Notes Prolactin is a secreted anterior pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation during pregnancy. However, prolactin has also additional pleiotropic effects in both males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG. Tested applicationsSandwich ELISAmore details PlatformMicroplate Properties Storage instructionsStore at +4°C. Please refer to protocols. Components 1 x 96 tests 10X Prolactin Capture Antibody 2 x 300μl 10X Prolactin Detector Antibody 2 x 300μl 10X Wash Buffer PT 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 2 x 3ml Plate Seals 1 unit Prolactin Human Lyophilized Recombinant Protein 2 vials Sample Diluent 50BS 2 x 12ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml Research Areas Signal Transduction Growth Factors/Hormones Hormones Cancer Growth factors Hormones Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Growth factors and hormones ELISA kits RelevanceVarious hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation. Cellular localizationSecretedAlternative names AV290867 Lactogenic Hormone Luteotropic Hormone see allDatabase links Entrez Gene: 5617 Human Omim: 176760 Human SwissProt: P01236 Human Unigene: 1905 Human Applications Our Abpromise guarantee covers the use of ab189570 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes Sandwich ELISA Use at an assay dependent concentration.