Product nameENO1 (Alpha-Enolase) Human SimpleStep ELISA? Kit See all ENO1 kitsDetection methodColorimetricPrecision Intra-assay Sample n Mean SD CV% HeLa extract 5 5.7% Inter-assay Sample n Mean SD CV% HeLa extract 3 4.7% Sample typeSerum, Cell culture extracts, Tissue ExtractsAssay typeSandwich (quantitative)Sensitivity 8.1 U/mlRange12.5 U/ml - 800 U/mlRecovery 90.1 % Sample specific recovery Sample type Average % Range Serum 96.4 87.4% - 104.1% Cell culture media 99.5 97.2% - 102.7% Goat Serum 90.4 83.5% - 95.2% Assay time1h 30m Assay durationOne step assaySpecies reactivityReacts with: Human Product overview Abcam’s ENO1 (alpha-enolase) in vitro SimpleStep ELISA? (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ENO1 protein in Human serum, cell and tissue extracts. The SimpleStep ELISA? employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. Notes Alpha enolase (ENO1, P06733) ENO1 is localized to cytoplasm. It can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. MBP1, a shorter isoform of the ENO1, binds to the myc promoter and acts as a transcriptional repressor. It may be a tumor suppressor. It locates mainly in nucleus. ENO1 is used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also it is a marker for endometriosis. Antibodies against ENO1 are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. ENO1 is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism. Tested applicationsSandwich ELISAmore details PlatformMicroplate Properties Storage instructionsStore at +4°C. Please refer to protocols. Components 1 x 96 tests 10X ENO1 Capture Antibody 1 x 600μl 10X ENO1 Detector Antibody 1 x 600μl 10X Wash Buffer PT 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml ENO1 Human Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml Research Areas Immunology Immune System Diseases Allergens Signal Transduction Metabolism Energy Metabolism Cell Biology Cell Cycle Cell differentiation Cancer Cell cycle Cell differentiation Cancer Tumor biomarkers Other Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Cell cycle proteins ELISA kits Metabolism Pathways and Processes Metabolic signaling pathways Energy transfer pathways Energy Metabolism Cancer Cancer Biomarker Panels Biomarker Panels Cellular localizationCytoplasm. Cell membrane. Cytoplasm ? myofibril ? sarcomere ? M line. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.Alternative names 2-phospho-D-glycerate hydro-lyase alpha enolase like 1 alpha-enolase see allDatabase links Entrez Gene: 2023 Human Omim: 172430 Human SwissProt: P06733 Human Unigene: 517145 Human Applications Our Abpromise guarantee covers the use of ab181417 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes Sandwich ELISA Use at an assay dependent concentration.