国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

武漢艾美捷科技有限公司
初級會員 | 第6年

13797054889

中英文說明丨艾美捷 SiR-Actin試劑盒#CY-SC001

時間:2023/5/6閱讀:144
分享:

SiR-actin has fluorescence, cell permeability, and high specificity for F-actin. Sir-atin staining for endogenous F-actin does not require gene manipulation or overexpression. Its emission in far-red light minimizes phototoxicity and the self fluorescence of the sample. SiR-actin is compatible with GFP and/or m-Cherry fluorescent proteins. It can be imaged using standard Cy5 filters. SiR-actin can be used for wide field of view, confocal, SIM or STED imaging of living cells and tissues. The number of probes allows for 50 to 200 staining experiments* (* Based on the following conditions: 0.5 1ml staining solution/0.5 1um probe concentration for staining experiments. The number of staining experiments can be further increased by reducing the volume or probe concentration).

 

SiR Action cell imaging

The imaging of SiR action is best done using standard Cy5 settings. After labeling, live cells can be immediately imaged without the need for washing steps. Optionally, a simple washing step includes replacing the labeled solution with fresh culture medium without probes, which typically improves the signal-to-noise ratio. If time elapses for imaging, it is recommended to keep the probe concentration equal to or lower than 100 nM throughout the experiment to obtain a constant signal and avoid probe interference with actin dynamics (reducing cell proliferation). If cleaning cells

Before imaging, the staining will last for several hours.

 

SiR-actin具有熒光性、細(xì)胞通透性和對F-actin的高度特異性。Sir-actin染色內(nèi)源性的F-actin不需要基因操作或過表達(dá)。它在遠(yuǎn)紅光中的發(fā)射最大限度地降低了光毒性和樣品的自身熒光。SiR-actin兼容GFP和/或m-cherry熒光蛋白質(zhì)。它可以用標(biāo)準(zhǔn)的Cy5濾鏡進(jìn)行成像。SiR-actin可用于活細(xì)胞和組織的寬視場、共聚焦、SIM或STED成像。探針數(shù)量允許50 - 200染色實驗。*(*基于以下條件:0.5 - 1ml染色液/ 0.5 - 1um探針濃度的染色實驗。染色實驗的數(shù)量可以通過減少體積或探針濃度來進(jìn)一步增加)。

 

艾美捷 SiR-Actin試劑盒#CY-SC001物理特性:

abs 652 nm

Em 674 nm

ε652 nm 1.0·105 mol-1·cm-1

MW 1241.6 g/mol

MF C71H88N8O10Si

638189870923261923630.png

探針濃度(nM) 建議標(biāo)記時間(h)*

> 1000      0.5 - 1

500           3 - 4

200           4 - 6

< 100        6 - 12

 

SiR-Actin試劑盒文獻(xiàn)參考:

1. Fluorogenic probes for live-cell imaging of the cytoskeleton, G. Lukinavi?ius et al., Nature Methods, 11, 731–733 (2014)


會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~
撥打電話
在線留言
乐山市| 扎赉特旗| 新巴尔虎左旗| 内黄县| 临清市| 南溪县| 石城县| 衡阳市| 湄潭县| 登封市| 榆社县| 马山县| 津南区| 益阳市| 紫云| 凤阳县| 安丘市| 府谷县| 海兴县| 宜黄县| 双牌县| 庆城县| 台东市| 麟游县| 阜康市| 循化| 眉山市| 涞源县| 临江市| 东阳市| 桦甸市| 富蕴县| 神农架林区| 安徽省| 绥化市| 独山县| 彰武县| 乐都县| 辽源市| 韶关市| 会宁县|