MS culture medium was designed by Murashige and Skoog for tobacco cell culture in 1962. It is characterized by high concentration of inorganic salts and ions, and is a relatively stable ion balance solution. Its nitrate content is high, and the amount and proportion of nutrients are appropriate, which can meet the nutritional and physiological needs of plant cells. Therefore, it is widely used as the basic medium for rapid propagation of most plant tissue culture. Based on this, this culture medium was named after them.

MS medium is a very commonly used tissue culture medium, in which many variants have been developed. This culture medium originated from White's culture medium and was initially developed for the c*tion of tobacco callus tissue. Compared to white culture medium, the concentration of all components increased. Increasing nitrogen to 50-60mM significantly stimulates the growth of tobacco cells, but concentrations of 80mM or higher are clearly detrimental to the cells. The increase of all other elements, especially macro elements, also stimulates the growth of callus tissue. Due to the high concentration of minerals, MS medium is a very rich saline medium, which may be too salty for certain plant species. To avoid this issue, MS is usually used in conjunction with trace elements at full concentrations, but with constant elements at concentrations of half or three-quarters as originally described by the author. Sometimes, the original MS vitamins are replaced by vitamins from Linsmaier and Gamborg B5 culture media, as the concentration of thiamine is higher compared to the demand for this vitamin in plants.

MS培養(yǎng)基是Murashige和Skoog于1962年為煙草細(xì)胞培養(yǎng)設(shè)計(jì)的，其特點(diǎn)是無(wú)機(jī)鹽和離子濃度較高，是較穩(wěn)定的離子平衡溶液，它的硝酸鹽含量高，其養(yǎng)分的數(shù)量和比例合適，能滿足植物細(xì)胞的營(yíng)養(yǎng)和生理需要，因而適用范圍比較廣，多數(shù)植物組織培養(yǎng)快速繁殖用它作為培養(yǎng)基的基本培養(yǎng)基。 基于此，這種培養(yǎng)基就用他們的名字來命名了。

MS培養(yǎng)基是非常常用的組織培養(yǎng)基，其中已經(jīng)開發(fā)了許多變體。該培養(yǎng)基起源于懷特的培養(yǎng)基，最初是為種植煙草愈傷組織而開發(fā)的。與白色培養(yǎng)基相比，所有成分的濃度都增加了。增加至50-60mM氮顯著刺激煙草細(xì)胞的生長(zhǎng)，然而濃度為80mM或更高顯然對(duì)細(xì)胞不利。所有其他元素的增加，特別是宏觀元素的增加，也刺激了愈傷組織的生長(zhǎng)。由于礦物質(zhì)濃度高，MS培養(yǎng)基是一種非常豐富的鹽水培養(yǎng)基，對(duì)某些植物物種來說可能太咸了。為了避免這個(gè)問題，MS通常與全濃度的微量元素一起使用，但與作者最初描述的濃度的一半或四分之三的常量元素一起使用。有時(shí)，原始的MS維生素被Linsmaier和Gamborg B5培養(yǎng)基的維生素所取代，因?yàn)橄鄬?duì)于植物對(duì)這種維生素的需求，硫胺素的濃度較高。

艾美捷 Murashige & Skoog培養(yǎng)基含維生素#M0222.0050組成：

微量元素 毫克/升 微米

氯化鈷2.6H2O 0.025 0.11

銅酸4.5H2O 0.025 0.10

菲納多達(dá) 36.70 100.00

H3博3 6.20 100.27

基 0.83 5.00

錳硫4.H2O 16.90 100.00

那2哞4.2H2O 0.25 1.03

氧化鋅4.7H2O 8.60 29.91

宏元素 毫克/升 毫米

氯化鈣2 332.02 2.99

韓2采購(gòu)訂單4 170.00 1.25

諾3 1900.00 18.79

鎂硫磺4 180.54 1.50

新罕布丨什爾州4不3 1650.00 20.61

維生素 毫克/升 微米

甘氨酸 2.00 26.64

肌醇肌醇 100.00 554.94

煙酸 0.50 4.06

鹽酸吡哆醇 0.50 2.43

鹽酸硫胺素 0.10 0.30

包括維生素在內(nèi)的微觀和宏觀元素總濃度：4405.19毫克/升

FORMULA,INGREDIENTS     PER     LITER  

Potassium Nitrate 1900.00 mg/I Batch number :017465

Ammonium  Nitrate 1650.00 mg/1 Batch number :017160

Potassium Dihydrogen Phosphate 170.00  mg/ Batch  number:017087

Magnesium Sulphate Anhydrous   180.54 mg/1 Batch number  :017230

MS Micro Elements (M0301)   69.53 mg/l Batch number :P21196

Calcium Chloride Anhydrous   332.02 mg/l Batch number :017261

MS Vitamin Mixture (M0409)   103.10 mg/l Batch number :P20929