歡迎聯(lián)系我

有什么可以幫您？在線咨詢

DSS誘導(dǎo)斑馬魚潰瘍性結(jié)腸炎模型的建立

來(lái)源：翌圣生物科技（上海）股份有限公司 2024年07月15日 10:02

斑馬魚作為腸道損傷模型的應(yīng)用已得到充分證實(shí)，葡聚糖硫酸鈉（Dextran Sulfate Sodium Salt，DSS）誘導(dǎo)的斑馬魚IBD模型模擬了哺乳動(dòng)物IBD表型的不同特征，包括腸道中性粒細(xì)胞炎癥、過(guò)多粘液生成、腸淋巴管生成增加以及前炎性細(xì)胞因子上調(diào)。

圖片來(lái)源：包圖網(wǎng)

實(shí)驗(yàn)動(dòng)物

3dpf（days post fertilization) 斑馬魚

實(shí)驗(yàn)材料

DSS（Yeasen 60316ES，MW:36000~50000）

實(shí)驗(yàn)步驟

1. 將斑馬魚胚胎培養(yǎng)在含甲基藍(lán)的E3胚胎培養(yǎng)基中，28.5 ℃，培養(yǎng)至1 dpf；

2. 1dpf 后使用不含亞甲基藍(lán)的E3胚胎培養(yǎng)基，培養(yǎng)至3 dpf；

3. 用E3培養(yǎng)基配制濃度為10% DSS的儲(chǔ)存液;

4. 用培養(yǎng)基稀釋DSS至最大非致死劑量（DSS濃度參考：0.5%）；

5. 用0.5%的DSS處理斑馬魚，從3 dpf處理到6 dpf，觀察指標(biāo)[1]。

結(jié)果分析

造模成功指標(biāo)：中性粒細(xì)胞數(shù)量增多、酸性粘液蛋白增多、炎性因子表達(dá)量上升。

1.嗜中性粒細(xì)胞數(shù)量：中性粒細(xì)胞數(shù)量增多，并且向腸和上皮中移動(dòng)

2.酸性粘液蛋白：石蠟病理切片結(jié)合AB-PAS染色法驗(yàn)證，DSS浸泡后斑馬魚腸道中酸性粘液蛋白增多

3.炎性因子表達(dá)量：DSS處理后斑馬魚腸道中炎性因子表達(dá)量上升[1,2]

客戶案例

圖1.Yeasen DSS（Cat#60316ES）斑馬魚造模實(shí)驗(yàn)結(jié)果（Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557）

DSS處理組相較于對(duì)照組，酸性粘蛋白染色和粘液分泌增加，斑馬魚外周血中的嗜中性粒細(xì)胞數(shù)量增多并且向腸和上皮中移動(dòng)，炎性因子表達(dá)量顯著上調(diào)，腸道出現(xiàn)炎癥反應(yīng)。

翌圣DSS產(chǎn)品優(yōu)勢(shì)

翌圣提供高品質(zhì)DSS（Cat.NO：60316ES，MW:36000~50000），具有以下優(yōu)勢(shì)：

1.高純度（＞98%），硫含量17-19%，游離硫＜0.2%，大量數(shù)據(jù)文獻(xiàn)支持，廣泛應(yīng)用于UC模型的構(gòu)建；

2.多種屬動(dòng)物中均可造模：小鼠、大鼠、斑馬魚、豬、果蠅；

3.癥狀表現(xiàn)與人UC極度相似：可用于研究急、慢性結(jié)腸炎的發(fā)生發(fā)展機(jī)制，也可用于藥物的藥效研究；

4.簡(jiǎn)單易行，成模率高，重復(fù)性強(qiáng)；

5.聯(lián)合氧化偶氮甲烷 (azoxymethane, AOM)用藥，可用于誘發(fā)結(jié)腸炎相關(guān)性癌癥 (colitis associated cancer, CAC) 動(dòng)物模型，成功模擬IBD誘發(fā)CAC的過(guò)程。

建模效率與進(jìn)口產(chǎn)品一致，產(chǎn)品價(jià)格低至進(jìn)口價(jià)格的1/3，大量現(xiàn)貨庫(kù)存。

產(chǎn)品訂購(gòu)

產(chǎn)品名稱	貨號(hào)	規(guī)格	價(jià)格（元）	促價(jià)（元）
Dextran Sulfate Sodium Salt（DSS）結(jié)腸炎建模用葡聚糖硫酸鈉鹽?MW:36000~50000	60316ES25	25 g	1255	955

Dextran Sulfate Sodium Salt（DSS）結(jié)腸炎建模用葡聚糖硫酸鈉鹽?MW:36000~50000	60316ES60	100 g	3855	2655

Dextran Sulfate Sodium Salt（DSS）結(jié)腸炎建模用葡聚糖硫酸鈉鹽?MW:36000~50000	60316ES76	500 g	16855	9655

Dextran Sulfate Sodium Salt（DSS）結(jié)腸炎建模用葡聚糖硫酸鈉鹽?MW:36000~50000	60316ES80	1 kg	32855	18655

點(diǎn)擊產(chǎn)品名稱查看詳情

其他產(chǎn)品推薦

貨號(hào)	產(chǎn)品名稱	規(guī)格	應(yīng)用場(chǎng)景
18820ES	MolPure® Stool DNA Kit 糞便DNA提取試劑盒	5 T/50 T/200 T	能高效獲得優(yōu)質(zhì)的糞便DNA，有利于后續(xù)對(duì)腸道微生物的研究。
18528ES	MolPure® Mag48 Soil/Stool DNA Kit FA 磁珠法48孔土壤 /糞便DNA提取試劑盒FA（預(yù)封裝）	48 T	磁珠法提取樣本基因組DNA。
18526ES	MolPure®Mag Soil/Stool DNA Kit 磁珠法土壤/糞便DNA提取試劑盒	20 T/50 T/200 T	磁珠法提取樣本基因組DNA。
60524ES	Hematoxylin and Eosin Staining Kit 蘇木素伊紅(H&E)染色試劑盒	2×100 mL	用于免疫組化中組織切片，血涂片，骨髓切片的染色。
60403ES	Urine Fecal Occult Blood Test Kit 尿糞隱血檢測(cè)試劑盒(聯(lián)苯胺法)	100T	對(duì)慢性消化道出血，如：消化性潰瘍等的研究篩選均具有重要價(jià)值。
60534ES	AB-PAS染色液	6×50 mL/6×100 mL	可鑒別同一組織中的中性黏蛋白和酸性黏蛋白。

產(chǎn)品已發(fā)表文章(部分)

向下滑動(dòng)查看

2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

2021

[1] Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[2] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[3] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[5] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

BEFORE 2020

[1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973

[2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048

[3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868

[4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943